Éva Kárpáti PhD student   Email: patiev.bio(at)gmail.com     „Factor H and complement regulation” research group

Short CV.:

• PhD student, Biology Doctoral School, Eötvös Loránd University, Department of Immunology, MTA-ELTE "Lendület" Complement Research Group (2015-, Supervisor: Dr. Mihály Józsi)
• Biologist - Hungarian Academy of Sciences, Research Centre for Natural Sciences, Institute of Enzymology, Systems Biology of Reproduction Research Group (2014-2015)
• Biology MSc, Eötvös Loránd University, Molecular-, Immune- and Microbiology specialty (2014)
• Biology BSc, Eötvös Loránd University (2012)

Scientific achievements:

• 2017 - Outstanding Abstract Achievement Award, European Meeting On Complement In Human Diseases, 2017.09.10-12, Copenhagen

Research Interest:

• PhD: The analysis of the complement activation regulator factor H family's role in necrotizing/apoptotic and primer cells (monocytes, neutrophil granulocytes).
• BSc and MSc thesis: Investigating the relationship between the female sex hormone estrogen and the immune system. The identification of the membrane-associated estrogen receptor(s) and the SHBG (Sex Hormone Binding Globulin) as E2 transporting protein and its receptors on lymphoid cells, as well as clarification of  the functional role of SHBG.
• The maternal-fetal immunoregulation and its cellular and molecular components. The aims of the research group are the investigation of: 1) the immune interactions of the mother and her fetus at the molecular and cellular level; 2) the aspects of the maternal adaptive and innate immune responses against fetal antigens; and 3) the maternal and fetal signaling pathways, which are of paramount importance for healthy pregnancy; 4) as well as the clarification of the role and mechanism of action of the galectin protein family (in particular PP13) in normal and abnormal immunoregulatory processes.

Methods:

• Cell culturing methods (human and mouse T cell, B cell and monocyte cell lines, Sf9 insect cells), cell isolation from blood, creating cell cultures (monocytes, granulocytes)
• Protein: fluorescent immunocytochemical labeling, flow cytometry (Becton-Dickinson FACS Calibur, Becton-Dickinson FACS Aria III), Western blot, confocal microscopy (FRET imaging, colocalization, calcium signal imaging, Olympus Fluoview 500 CLSM), protein expression in Sf9 cells, protein purification, ELISA
• DNA: agarose gel electrophoresis, DNA isolation

Publikációs lista:

• Józsi M, Schneider AE, Kárpáti É, Sándor N. Complement factor H family proteins in their non-canonical role as modulators of cellular functions. Semin Cell Dev Biol. 2018 Jan 4. pii: S1084-9521(17)30136-2. PMID: 29305301.
• Karpati E, Cserhalmi M, Csincsi AI, Hajnal D, Papp A, Uzonyi B, Jozsi M. Interaction of factor H family proteins with DNA and dead cells: Implications for the regulation of opsonization. MOLECULAR IMMUNOLOGY 89: pp. 140-141. (2017). 16th European Meeting on Complement in Human Disease (EMCHD). Copenhagen, Dánia: 2017.09.08 - 2017.09.12.
• Csincsi ÁI, Szabó Z, Bánlaki Z, Uzonyi B, Cserhalmi M, Kárpáti É, Tortajada A, Caesar JJE, Prohászka Z, Jokiranta TS, Lea SM, Rodríguez de Córdoba S, Józsi M. FHR-1 Binds to C-Reactive Protein and Enhances Rather than Inhibits Complement Activation. J Immunol. 2017 Jul 1; 199(1):292-303. PMID: 28533443.
• Andrea E. Schneider, Noémi Sándor, Éva Kárpáti, Mihály Józsi. Complement Factor H modulates the activation of human neutrophil granulocytes and the generation of neutrophil extracellular traps. Molecular Immunology. 2016 Apr;72:37-48. PMID: 26938503.
• Nandor Gabor Than, Roberto Romero, Andrea Balogh, Eva Karpati, Salvatore Andrea Mastrolia, Orna Staretz-Chacham, Sinuhe Hahn, Offer Erez, Zoltan Papp, Chong Jai Kim. Galectins: double edged swords in the cross-roads of pregnancy complications and female reproductive tract inflammation and neoplasia. Journal of Pathology and Translational Medicine. 2015 May;49(3):181-208.
• Than NG, Balogh A, Romero R, Kárpáti E, Erez O, Szilágyi A, Kovalszky I, Sammar M, Gizurarson S, Matkó J, Závodszky P, Papp Z, Meiri H. Placental Protein 13 (PP13) - A Placental Immunoregulatory Galectin Protecting Pregnancy. Front Immunol. 2014 Aug 20;5:348. doi: 10.3389/fimmu.2014.00348. eCollection 2014. Review. PubMed PMID: 25191322; PubMed Central PMID: PMC4138504.
• Schneider AE, Kárpáti E, Schuszter K, Tóth EA, Kiss E, Kulcsár M, László G, Matko J. A dynamic network of estrogen receptors in murine lymphocytes: fine-tuning the immune response. J Leukoc Biol. 2014 Nov;96(5):857-72. doi: 10.1189/jlb.2A0214-080RR. Epub 2014 Jul 28. PubMed PMID: 25070950.

Conference abstracts:

• MIT, Bükfürdő, 2018. Éva Kárpáti, Andrea E. Schneider, Noémi Sándor, Dávid Hajnal, Mihály Józsi
  Complement factor H family proteins influence monocyte and neutrophil cell functions.
• MESIA, Budapest, 2016.12.1-3; EMCHD, Copenhagen, 2017.09.10-12., MIT, Velence, 2017. Éva Kárpáti, Marcell Cserhalmi, Ádám I. Csincsi, Dávid Hajnal, Alexandra Papp, Barbara Uzonyi, Mihály Józsi
  Interaction of factor H family proteins with DNA and dead cells: implications for regulation of opsonization.
• MIT, Velence, 2016. Éva Kárpáti, Marcell Cserhalmi, Ádám I. Csincsi, Dávid Hajnal, Barbara Uzonyi, Mihály Józsi
  Interaction of Factor H protein family members with DNA and dead cells, and implications for regulation of opsonization.
• IMPULSE, Mátraháza, 2013.08.31-09.03. E. Schneider Andrea, Kárpáti Éva, Schuszter Kitti, László Glória, Matkó János: Membrane estrogen receptor(s) on lymphocytes: the linkage between the genomial and non-genomial pathways. Immun-related Phatologies: Understanding Leukocyte Signaling and Emerging Therapies. 2013. augusztus 31 - szeptember 3. Mátraháza, Magyarország.